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ISOLASI DAN IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DARI SPONS Callyspongia sp. SERTA UJI AKTIVITASNYA SEBAGAI ANTIBAKTERI
Abdul Arif Rachmat Halim
F1F1 13 145
ABSTRAK
Telah dilakukan isolasi dan identifikasi senyawa metabolit sekunder dari spons Callyspongia sp. serta uji aktivitasnya terhadap antibakteri. Pemisahan senyawa kimia dari ekstrak spons dilakukan dengan teknik kromatografi caik vakum (KCV), kromatografi radial (KR), dan kromatografi lapis tipis (KLT). Dua senyawa berhasil diisolasi, hasil analisis data spektroskopi NMR-1D (1H, 13C-NMR) serta perbandingan data sejenis dari literatur menunjukkan bahwa senyawa isolat C1 dan C2 aadalah kolesterol dan senyawa golongan alkaloid lainnya. Pengujian aktivitas antibakteri menggunakan teknik mikrodilusi. Ekstrak Callyspongia sp. tidak aktif sebagai antibakteri terhadap bakteri Bacillus subtilis, Salmonella enterica, Streptococcus mutans, Escherichia coli. Isolat C1 dan C2 menunjukkan aktivitas penghambatan pada bakteri Salmonella enterica pada konsentrasi hambat minimum 128 dan 256 μg/mL.
Kata kunci: Antibakteri, isolasi, spons, Callyspongia sp.
ISOLATION ANF IDENTIFICATION OF SECONDARY METABOLITE COMPOUNDS FROM SPONGES OF Callyspongia sp. AND ITS ACTIVITIES AS ANTIBACTERIAL
Abdul Arif Rachmat Halim
F1F1 13 145
ABSTRACT
Isolation and identification of secondary metabolites from sponges Callyspongia sp. as well as antibacterial activity of all metabolites have been carried out. The separation of chemical compounds from sponges extract was carried out by vacuum liquid chromatography, radial chromatography and Thin Layer Chromatography (TLC) techniques. Two compounds was isolated, analysis of NMR-1D spectroscopic data (1H, 13C-NMR) and comparison of similar data from the literature showed that compounds C1 and C2 isolates was cholesterol and other alkaloid compounds. Antibacterial activity testing uses the microdilution technique. Callyspongia sp. extract not active as an antibacterial against Bacillus subtilis, Salmonella enterica, Streptococcus mutans, Escherichia coli. C1 and C2 isolates showed inhibitory activity in Salmonella enterica bacteria at a minimum inhibitory concentration of 128 and 256 μg / mL.
Keywords: Antibacterial, isolation, sponge, Callyspongia sp.
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